Title: Mesoscopic Oblique Plane Microscopy (Meso-OPM) platform for Multi-scale and Multi-Photon Volumetric Dynamic Imaging

Meso-OPM leverages key advantages of traditional light-sheet microscopy while adding high-speed dynamic imaging over a mesoscopic scale. Here I present a 2P Meso-OPM system with switchable FOV and resolution to achieve >5Hz volumetric rate imaging across multi-millimeter lateral FOVs and multi-layer cortical depth penetration. I first demonstrate the systems preliminary success with one-photon excitation to capture calcium dynamics across the larval zebrafish body during the optomotor response. Moving forward, I will leverage this system with more complex mammalian models such as mice. I will integrate a two-photon excitation source for improved optical sectioning in this highly scattering tissue and capture large FOV, calcium rate neural responses during sensory activation of the barrel cortex. This will provide high spatiotemporal calcium fluorescence information with a multi-millimeter FOV across multiple cortical layers in mammalian cortex and provide a robust tool for understanding sensory circuitry in the brain.